Somatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion.
نویسندگان
چکیده
As an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transgenic strains that are hygromycinor G418-resistant by selecting hybrids on medium containing both antibiotics.
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2009 2 شماره
صفحات -
تاریخ انتشار 2009